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- Title
Chelerythrine Attenuates Renal Ischemia/Reperfusion-induced Myocardial Injury by Activating CSE/H<sub>2</sub>S via PKC/NF-κB Pathway in Diabetic Rats.
- Authors
Hu, Bo; Xu, Guangtao; Zheng, Yongxia; Tong, Fei; Qian, Ping; Pan, Xiaoyan; Zhou, Xinmei; Shen, Ruilin
- Abstract
Background/Aims: Chelerythrine (CHE), a benzophenanthridine alkaloid, is a potent, selective, and cell-permeable protein kinase C (PKC) inhibitor. The purpose of the present study was to evaluate the effect of CHE on myocardial recovery after renal ischemia/reperfusion (I/R)-induced myocardial injury (RI/RMI) in a streptozocin (STZ)-induced diabetic rat model. Methods: Diabetes mellitus (DM) rats preconditioned with CHE and D, L-propargylglycine (PAG) were subjected to renal I/R. The extent of cardiac morphologic lesions and the biochemical markers of cardiorenal function and oxidative stress were detected utilizing hematoxylin-eosin staining, commercial kits, and enzyme-linked immunoassay, respectively. The expressions of cystathionine-γ-lyase (CSE), PKC-α, PKC-β2, and nuclear factor-kappa B (NF-κB) in the rat myocardial tissue were measured utilizing western blotting. Results: Renal I/R treatment resulted in myocardial injury. CHE-preconditioning promoted the recovery from myocardial damage by ameliorating the biochemical parameters of myocardial injury, reducing oxidative stress, increasing the H2S level, up-regulating the expression of CSE, and down-regulating the expressions of PKC-α, PKC-β2, and NF-κB. Conclusion: These findings suggest that CHE-pretreatment may exert a protective effect on the myocardium against RI/RMI by activating endogenous CSE/H2S via PKC/NF-κB pathway in STZ-induced diabetic rats. Further studies are needed defining underlying mechanisms.
- Subjects
ISCHEMIA diagnosis; ISCHEMIA treatment; REPERFUSION injury; MICE behavior; LABORATORY mice; CELL permeability; PROTEIN kinase C genetics
- Publication
Kidney & Blood Pressure Research, 2017, Vol 42, Issue 2, p379
- ISSN
1420-4096
- Publication type
Article
- DOI
10.1159/000477948