We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Morpho-Molecular Diagnosis of Papillary Thyroid Carcinoma: BRAFV600E Detection as an Important Tool in Preoperative Evaluation of Fine-Needle Aspirates.
- Authors
Ivo Marchetti; Francesca Lessi; Chiara Maria Mazzanti; Gloria Bertacca; Rossella Elisei; Giancarlo Di Coscio; Aldo Pinchera; Generoso Bevilacqua
- Abstract
Background:Although most thyroid nodule fine-needle aspiration (FNA) diagnoses are definitive or nearly definitive, about 30% of them are not read as definitively benign or malignant, the so-called indeterminate or suspicious FNA diagnosis. The prevalence of malignancy in FNA samples with these diagnoses varies from 10% to 52%. The first aim of this study was to determine if BRAFV600E analysis of thyroid FNA cytological smears could be performed with a relatively simple protocol. We also sought to determine if assessing the presence of BRAFgene mutations in preoperative FNA cytology slides would provide diagnostic information for FNA samples with a reading of indeterminate or suspicious thyroid lesions.Methods:DNA was extracted directly from FNA-stained smears of 111 patients with thyroid lesions having different cytological diagnoses. There was 1 cystic nodule, 20 microfollicular proliferations without atypia, 32 that were suspicious for papillary carcinoma, 56 papillary thyroid carcinomas (PTC), and 2 poorly differentiated carcinomas. The BRAFV600E mutational status was determined by sequencing analysis in all patients. The histopathological diagnosis was obtained in all cases.Results:We observed that 56/90 (62.3%) patients received a definitive diagnosis of PTC when only cytology was used. After molecular analysis, the BRAFV600E mutation was detected in 18/32 (56.2%) cases with a cytology of suspicious for papillary carcinoma and 41/56 (73.2%) with PTC. According to the morpho-molecular analysis (i.e., traditional cytology combined with BRAFV600E analysis) 74/90 (82.2%) patients could be assigned a definitive diagnosis of PTC. Therefore, the addition of molecular analysis yielded an increase of 20% in the sensitivity compared to cytology alone.Conclusions:The method of molecular analysis of thyroid FNA smears described here can be easily performed after the FNA, thereby avoiding inconvenience and additional time during the FNA and permitting later analysis of samples having indeterminate cytology features. The increased sensitivity of this preoperative morpho-molecular analysis should provide information that is useful in deciding the extent of thyroid surgery for thyroid nodules that are indeterminate or suspicious on cytology.
- Subjects
MOLECULAR diagnosis; THYROID cancer diagnosis; PAPILLARY carcinoma; PREOPERATIVE care; NEEDLE biopsy; GENETIC mutation; DISEASE prevalence; MEDICAL protocols
- Publication
Thyroid, 2009, Vol 19, Issue 8, p837
- ISSN
1050-7256
- Publication type
Article
- DOI
10.1089/thy.2009.0074