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- Title
Screening of ethyl acetate extract of Bridelia micrantha for hepatoprotective and anti-oxidant activities on Wistar rats
- Authors
Nwaehujor, Chinaka O; Udeh, Nkeiruka E
- Abstract
Abstract: Objective: To explore the hepatoprotective and anti-oxidant activities of the methanolic leaf extract of Bridelia micrantha (B. micrantha) on paracetamol induced liver damage in Wistar rats. Methods: Parameters were measured including alanine aminotransaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and total protein. The anti-oxidant effects were studied using the 1, 1-Diphenynl-2-Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assay methods. Results: B. micrantha extract decreased the level of AST in the rats given PCM from (129.47±0.92I) IU/L to (57.78±1.71) IU/L (P<0.05). This was lower than the value for Silymarin which was (59.92±1.41) IU/L. ALT concentration was reduced from (150.18±2.23) IU/L to (79.10±2.01) IU/L (P<0.05). ALP was reduced from (49.86±0.85) IU/L to (29.64±1.53) IU/L (P<0.05). Total bilirubin was reduced from (2.14±0.10 mg/dL) to (0.18±0.07) mg/dL (P<0.05) while total protein was increased from (4.26±0.30) mg/dL to (6.20±0.19) mg/dL (P<0.05). Concentrations ranging from 10 – 400 μg/mL of B. micrantha were assayed for antioxidant activities. The DPPH assay showed 98% antioxidant activity at concentration of 400 μg/mL. The FRAP values were 0.016, 0.39, 0.455, 0.601 and 1.382 μM at 10, 50, 100, 200 and 400 μg/mL respectively. Conclusions: Results suggest that B. micrantha has hepatoprotective and anti oxidant potentials. However, further work involving fractionation needs to done to isolate the active compound responsible for the hepatoprotective activity.
- Subjects
ACETATES; ANTIOXIDANTS; ANGIOSPERMS; PLANT extracts; LEAVES; LABORATORY rats; ALKALINE phosphatase
- Publication
Asian Pacific Journal of Tropical Medicine, 2011, Vol 4, Issue 10, p796
- ISSN
1995-7645
- Publication type
Article
- DOI
10.1016/S1995-7645(11)60196-X