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- Title
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6.
- Authors
Choi, Tae-Rim; Park, Ye-Lim; Song, Hun-Suk; Lee, Sun Mi; Park, Sol Lee; Lee, Hye Soo; Kim, Hyun-Joong; Bhatia, Shashi Kant; Gurav, Ranjit; Choi, Kwon-Young; Lee, Yoo Kyung; Yang, Yung-Hun; Kovalcik, Adriana
- Abstract
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (Tg) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 104, weight average molecular weight of 9.1 × 104, and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low Tg, and low crystallinity, indicating its potential use in medical applications.
- Subjects
POLYHYDROXYALKANOATES; MOLECULAR weights; PSEUDOMONAS; GEL permeation chromatography; GLASS transition temperature; DIFFERENTIAL scanning calorimetry; RHAMNOLIPIDS
- Publication
Polymers (20734360), 2021, Vol 13, Issue 9, p1398
- ISSN
2073-4360
- Publication type
Article
- DOI
10.3390/polym13091398