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- Title
A Rapid Semiautomatical Enzyme Linked Immunoassay Identifying Intercellular Adhesion Molecule-1 (ICAM-1) on the Alveolar Macrophage Surface.
- Authors
Kamp, Sabine; Kreft, B.; Braun, J.; Dalhoff, K.
- Abstract
Intercellular adhesion molecule-1 (ICAM-1), a member of the immunoglobulin gene superfamily, is a cytokine-inducible adhesion molecule, which plays a central role in leukocyte migration into sites of acute or chronic inflammation. In this article we describe a sandwich immunoenzymometric method which allows rapid, semiquantitative (in "enzyme immunoassay units", EU) identification of ICAM-1 on the surface of alveolar macrophages. We evaluated this method in two groups of patients with pulmonary sarcoidosis (n = 12) or bacterial pneumonia (n = 11) and a group of healthy volunteers (n = 6), comparing the results with those obtained by immunocytochemical staining. ICAM-1 expression on the sarcoid alveolar macrophages surface was significantly elevated, s compared with control alveolar macrophages (0.76 EU ± 0.27 vs. 0.44 EU ± 0.12, p < 0.01). ICAM- 1 expression on the surface of alveolar macrophages from patients with pneumonia was not elevated (0.48 EU ± 0.35). Stimulation with tumour necrosis factor-α (TNF-α) or interferon-γ (100 kU/1) led to a significant induction of ICAM-1 on the surface of control alveolar macrophages (0.76 EU ± 0.18, p < 0.005 for TNF-α, 0.64 EU ± 0.10, p < 0.005 for interferon-γ), whereas alveolar macrophages from both patient groups did not respond to cytokines even at high dosages. ICAM-1 expression on the surface of alveolar macrophages from patients with sarcoidosis correlated with the spontanepus release of TNF-α by macrophages (R = 0.77, p < 0.05). To summarize, the evaluation of ICAM-1 levels on the surface of cells harvested from the pulmonary compartment is a useful tool for interpreting the mechanisms leading to leukocyte accumulation and activation in inflammatory disorders of the lung.
- Publication
Clinical Chemistry & Laboratory Medicine, 1994, Vol 32, Issue 6, p455
- ISSN
1434-6621
- Publication type
Article