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- Title
An anatomical training model for cerebrospinal liquid collection and myelography in embalmed dog cadavers.
- Authors
Chiarelo Zero, Raphael; Piazzon de Souza Queiroz, Andrea Barros; Pereira Toscano, Cauê; Herreira Jarrouge, Daniel; Salvitti de Sá Rocha, Thiago André; Vedovelli Cardozo, Marita; Singaretti de Olieveira, Fabricio
- Abstract
In research and academic activities, guidelines are essential and imperative especially on the use of animals. Alternative methods that do not bring academic or scientific harm should also be sought. This study aimed to develop a training model for the collection of cerebrospinal fluid (CSF) and myelography in the cervical and lumbar regions in cadavers of embalmed dogs, using an alcoholic solution and curing salts for fixation and conservation. The dogs were divided into 4 grups of 8 animal each and stored between 2°C and 6°C, for 30, 60, 90, or 120 days. Durotomy was performed to implant two urethral catheters (one in the cranial direction and another in the caudal direction to the spinal cord access site), in the subduraracnoid space. This space was fixed via manual infusion of saline solution with a 20-mL syringe to simulate the presence of the CSF and the positive pressure, while the puncture was made. Four cadavers of each group were randomly selected for the CSF puncture from the atlantooccipital joint and in the lumbar region between L5 and L6, respectively, and four were used for CSF puncture training, in which radiographic contrast (myelography) was injected in the same locations. This model was cost-effective, did not utilize toxic products, and can preserve cadavers for up to 120 days. In this novel anatomical model, a maximum of 15 students can be trained on CSF puncture, allowing cervical and lumbar myelography and at least 30 perforations per cadaver.
- Subjects
HUMAN anatomical models; MYELOGRAPHY; MEDICAL cadavers; ATLANTO-occipital joint; LUMBOSACRAL region; URINARY catheters; URETHRA
- Publication
Brazilian Journal of Veterinary Science / Revista Brasileira de Ciência Veterinária, 2022, Vol 29, Issue 4, p175
- ISSN
1413-0130
- Publication type
Article
- DOI
10.4322/rbcv.2022.0031