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- Title
Use of Multiple Diagnostic Tests to Detect Mycobacterium pinnipedii Infections in a Large Group of South American Sea Lions (Otaria flavescens).
- Authors
Jurczynski, Kerstin; Lyashchenko, Konstantin P.; Scharpegge, Julia; Fluegger, Michael; Lacave, Geraldine; Moser, Irmgard; Tortschanoff, Sonia; Greenwald, Rena
- Abstract
Since 2000, Heidelberg Zoo has been dealing with tuberculosis caused by Mycobacterium pin-nipedii within its collection of South American sea lions (Otaria flavescens). Recently, more cases became known all across Europe. Various diagnostic methods, including microscopy, PCR, and culture of sputum samples; three serological tests (ElephantTB STAT-PAK® assay, multianti-gen print immunoassay [MAPIA], and dual path platform assay [DPP]); and diagnostic imaging, were used to examine 14 animals. M. pinnipe-dii infection was strongly suspected antemortem based on the diagnostic results and was confirmed at necropsy in 10 sea lions. ElephantTB STAT-PAK® assay, MAPIA, and DPP test showed the diagnostic potential for rapid detection of this dis-ease in live sea lions. The highest sensitivity was achieved when applying more than one test.
- Subjects
MYCOBACTERIAL disease diagnosis; POLYMERASE chain reaction; DIAGNOSTIC imaging; OTARIA flavescens; SEA lions; SOUTH Americans; DISEASES
- Publication
Aquatic Mammals, 2012, Vol 38, Issue 1, p43
- ISSN
0167-5427
- Publication type
Article
- DOI
10.1578/AM.38.1.2012.43