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- Title
Tubulin-blocked state of VDAC studied by polymer and ATP partitioning
- Authors
Gurnev, Philip A.; Rostovtseva, Tatiana K.; Bezrukov, Sergey M.
- Abstract
Abstract: Recently reported functional interaction between voltage-dependent anion channel of the outer mitochondrial membrane, VDAC, and dimeric tubulin is observed as a reversible channel blockage. Using partitioning of poly-(ethylene glycol)s of different molecular weights and reversal potential measurements, we probe the size and ion selectivity of the fully open and tubulin-blocked states of VDAC reconstituted into planar lipid bilayers. While the effective radius of the channel decreases by only a factor of 1.34±0.15, the selectivity reverses from initially anionic to cationic. Directly measuring ATP partitioning we demonstrate that these changes prohibit ATP from entering the channel in its tubulin-blocked state.
- Subjects
TUBULINS; ION channels; POLYMERS; ADENOSINE triphosphate; POLYETHYLENE glycol; MITOCHONDRIAL membranes; MOLECULAR weights
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2363
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.06.008