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- Title
Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells
- Authors
Sato, Sei-ichi; Inoue, Hiroki; Kogure, Takeshi; Tagaya, Mitsuo; Tani, Katsuko
- Abstract
Abstract: Mammals have three members of the intracellular phospholipase A1 protein family (phosphatidic acid preferring-phospholipase A1, p125, and KIAA0725p). In this study, we showed that KIAA0725p is localized in the Golgi, and is rapidly cycled between the Golgi and cytosol. Catalytic activity is important for targeting of KIAA0725p to Golgi membranes. RNA interference experiments suggested that KIAA0725p contributes to efficient membrane trafficking from the Golgi apparatus to the plasma membrane, but is not involved in brefeldin A-induced Golgi-to-endoplasmic reticulum retrograde transport. Structured summary: MINT-8019765: KIAA0725 (uniprotkb:O94830) and Beta-COP (uniprotkb:P53618) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-8019775: KIAA0725 (uniprotkb:O94830) and GM130 (uniprotkb:Q5PXD5) colocalize (MI:0403) by fluorescence microscopy (MI:0416)
- Subjects
PHOSPHOLIPASES; GALACTOSYLTRANSFERASES; ENDOGLYCOSIDASES; GOLGI apparatus; GREEN fluorescent protein; MONOCLONAL antibodies; ENDOPLASMIC reticulum; PHOSPHATIDIC acids
- Publication
FEBS Letters, 2010, Vol 584, Issue 21, p4389
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.09.047