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- Title
Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism
- Authors
Bleicher, Lucas; de Moura, Patricia Ribeiro; Watanabe, Leandra; Colau, Didier; Dumoutier, Laure; Renauld, Jean-Christophe; Polikarpov, Igor
- Abstract
Abstract: Interleukin-22 (IL-22) is a member of the interleukin-10 cytokine family, which is involved in anti-microbial defenses, tissue damage protection and repair, and acute phase responses. Its signaling mechanism involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain. We report a 1.9Å crystal structure of the IL-22/IL-22R1 complex, revealing crucial interacting residues at the IL-22/IL-22R1 interface. Functional importance of key residues was confirmed by site-directed mutagenesis and functional studies. Based on the X-ray structure of the binary complex, we discuss a molecular basis of the IL-22/IL-22R1 recognition by IL-10R2. Structured summary: MINT-6693956: Il22 (uniprotkb:Q9GZX6) and IL22R1 (uniprotkb:Q8N6P7) bind (MI:0407) by X-ray crystallography (MI:0114)
- Subjects
INTERLEUKIN-10; CYTOKINES; DIMERS; MUTAGENESIS; X-ray crystallography
- Publication
FEBS Letters, 2008, Vol 582, Issue 20, p2985
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.07.046