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- Title
An Arabidopsis cytochrome b561 with trans-membrane ferrireductase capability
- Authors
Bérczi, Alajos; Su, Dan; Asard, Han
- Abstract
Abstract: Ascorbate-reducible cytochromes b561 (Cyts-b561) are a class of intrinsic trans-membrane proteins. Tonoplast Cyt-b561 (TCytb), one of the four Cyt-b561 isoforms in Arabidopsis was localized to the tonoplast. We demonstrate here that the optical spectra, EPR spectra and redox potentials of recombinant TCytb are similar to those of the well characterized bovine chromaffin granule Cyt-b561. We provide evidence for the reduction of ferric-chelates by the reduced TCytb. It is also shown that TCytb is capable of trans-membrane electron transport from intracellular ascorbate to extracellular ferric-chelates in yeast cells.
- Subjects
PROTEINS; SPECTRUM analysis; MEMBRANE proteins; ARABIDOPSIS
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1505
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.03.006