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- Title
Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells
- Authors
Deshpande, Alina; Hammon, Rebecca J.; Sanders, Claire K.; Graves, Steven W.
- Abstract
Abstract: We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined with the function of such cells, this suggests that PA receptors interact with the extracellular matrix and that differentiation increases the number of PA-specific receptors, which supports previously observed differentiation-induced LT susceptibility. Our results quantifiably confirm that the generality of PA binding will complicate its use as a tumor targeting agent.
- Subjects
BACTERIAL diseases; KILLER cells; GRANULOCYTES; EXTRACELLULAR matrix
- Publication
FEBS Letters, 2006, Vol 580, Issue 17, p4172
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.06.070