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- Title
Apical cell adhesion molecule, trophinin, localizes to the nuclear envelope
- Authors
Aoyama, Junya; Nakayama, Yuji; Sugiyama, Daijiro; Saburi, Sakura; Nadano, Daita; Fukuda, Michiko N.; Yamaguchi, Naoto
- Abstract
Abstract: Trophinin mediates homophilic and apical cell adhesion between trophoblastic cells and endometrial epithelial cells, which is potentially the initial attachment step in human embryo implantation. Since trophinin is an atypical membrane protein without the signal sequence, it is possible that trophinin localizes to the cytoplasm. By treating trophinin-expressing trophoblastic cells with a series of detergents, we found significant levels of endogenous trophinin in the cytoplasm, particularly at the nuclear envelope (NE). Fluorescence photobleaching of GFP-trophinin expressed in COS-1 cells showed the stable association of trophinin with the NE, suggesting an additional role of trophinin besides apical cell adhesion.
- Subjects
NUCLEAR membranes; EPITHELIAL cells; MEMBRANE proteins; CYTOPLASM
- Publication
FEBS Letters, 2005, Vol 579, Issue 28, p6326
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.10.012