We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The longin domain regulates subcellular targeting of VAMP7 in Arabidopsis thaliana
- Authors
Uemura, Tomohiro; Sato, Masa H.; Takeyasu, Kunio
- Abstract
Abstract: SNAREs (soluble N-ethyl-maleimide sensitive factor attachment protein receptors) which locate on the specific organelle membrane assure the correct vesicular transport by mediating specific membrane fusions. SNAREs are referred to as R- or Q-SNAREs on the basis of the amino acid sequence similarities and specific conserved residues. All of the Arabidopsis R-SNAREs have a N-terminal domain, called the longin domain (LD). In this study, we investigated the vacuolar targeting mechanism of Arabidopsis R-SNAREs. The vacuolar localized AtVAMP711 was used as the mother protein of GFP-tagged chimeric proteins joined to several domains such as the LD, the SNARE motif (SNM) and the transmembrane domain (TMD) of other organelle-localized R-SNAREs. The results showed that, whereas the TMD is not relevant for the vacuolar targeting, a complete LD is essential for the vacuolar and subcellular targeting.
- Subjects
GREEN fluorescent protein; ARABIDOPSIS; CELL membranes; PROTEIN analysis
- Publication
FEBS Letters, 2005, Vol 579, Issue 13, p2842
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.04.022