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- Title
Development of a recombinant anti-Vel immunoglobulin M to identify Vel-negative donors.
- Authors
van der Rijst, Marea V.E.; Lissenberg‐Thunnissen, Suzanne N.; Ligthart, Peter C.; Visser, Remco; Jongerius, John M.; Voorn, Lesley; Veldhuisen, Barbera; Vidarsson, Gestur; van den Akker, Emile; van der Schoot, C. Ellen; Lissenberg-Thunnissen, Suzanne N
- Abstract
<bold>Background: </bold>Alloimmunization against the high-frequency Vel blood group antigen may result in transfusion reactions or hemolytic disease of fetus and newborn. Patients with anti-Vel alloantibodies require Vel-negative blood but Vel-negative individuals are rare (1:4000). Identification of Vel-negative donors ensures availability of Vel-negative blood; however, accurate Vel blood group typing is difficult due to variable Vel antigen expression and limited availability of anti-Vel typing sera. We report the production of a recombinant anti-Vel that also identifies weak Vel expression.<bold>Study Design and Methods: </bold>A recombinant anti-Vel monoclonal antibody was produced by cloning the variable regions from an anti-Vel-specific B cell isolated from an alloimmunized patient into a vector harboring the constant regions of immunoglobulin (Ig)G1-kappa or IgM-kappa. Antibody Vel specificity was tested by reactivity to SMIM1-transfected HEK293T cells and by testing various red blood cells (RBCs) of donors with normal, weak, or no Vel expression. High-throughput donor screening applicability was tested using an automated blood group analyzer.<bold>Results: </bold>A Vel-specific IgM class antibody was produced. The antibody was able to distinguish between Vel-negative and very weak Vel antigen-expressing RBCs by direct agglutination and in high-throughput settings using a fully automated blood group analyzer and performed better than currently used human anti-Vel sera. High-throughput screening of 13,288 blood donations identified three new Vel-negative donors.<bold>Conclusion: </bold>We generated a directly agglutinating recombinant anti-Vel IgM, M3F5S-IgM, functional in manual, automated agglutination assays and flow cytometry settings. This IgM anti-Vel will improve diagnostics by facilitating the identification of Vel-negative blood donors.
- Subjects
ROYAL Bank of Canada; BLOOD group antigens; BLOOD group incompatibility; BLOOD groups; ERYTHROCYTES; ERYTHROBLASTOSIS fetalis; MONOCLONAL antibodies
- Publication
Transfusion, 2019, Vol 59, Issue 4, p1359
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.15147