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- Title
Ultrastructural localization of adhalin in normal murine skeletal myofiber.
- Authors
Wakayama, Yoshihiro; Inoue, Masahiko; Murahashi, Makoto; Shibuya, Seiji; Jimi, Takahiro; Kojima, Hiroko; Oniki, Hiroaki
- Abstract
The ultrastructural localization of adhalin and its relations to dystrophin, β-dystroglycan, and β-spectrin were studied in normal murine skeletal myofibers. The C-terminal peptides of adhalin and β-dystroglycan were synthesized based on their cDNAs, and the affinity-purified antibodies against these peptides were produced. Single-immunolabeling electron microscopy showed that the adhalin was located just inside the muscle plasma membrane or inside the myofiber a short distance from the plasma membrane. The adhalin signal was also noted at the sarcoplasmic side of plasmalemmd invaginations or at vesicular structures in subsarcolemmal areas. Double-immunogold-labeling electron microscopy disclosed a similar localization of dystrophin, β-dystroglycan, and β-spectrin. The close association of adhalin with dystrophin or β-dystroglycan was demonstrated by formation of doublets by signals of antibodies of adhalin with those of dystrophin or β-dystroglycan and was confirmed by statistical analyses. This study demonstrated that the location of adhalin is close to that of dystrophin and β-dystroglycan at the muscle plasma membrane.
- Publication
Annals of Neurology, 1996, Vol 39, Issue 2, p217
- ISSN
0364-5134
- Publication type
Article
- DOI
10.1002/ana.410390211