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- Title
Isolation and RFLP Genotyping of Toxoplasma gondii in Free-Range Chickens ( Gallus domesticus) in Grenada, West Indies, Revealed Widespread and Dominance of Clonal Type III Parasites.
- Authors
Chikweto, Alfred; Sharma, Ravindra N.; Tiwari, Keshaw P.; Verma, Shiv K.; Calero-Bernal, Rafael; Jiang, Tiantian; Su, Chunlei; Kwok, Oliver C.; Dubey, Jitender P.
- Abstract
The objectives of the present cross-sectional study were to isolate and genotype Toxoplasma gondii in free-range chickens from Grenada, West Indies. Using the modified agglutination test, antibodies to T. gondii were found in 39 (26.9%) of 145 free-range chickens with titers of 25 in 7 chickens, 50 in 6 chickens, 100 in 2 chickens, and 200 or higher in 24 chickens. The hearts of the 39 seropositive chickens were bioassayed in mice; viable T. gondii was isolated from 20 and further propagated in cell culture. Genotyping of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed 4 genotypes, including ToxoDB PCR-RFLP no. 2 (Type III), no. 7, no. 13, and no. 259 (new). These results indicated that T. gondii population genetics in free-range chickens seems to be moderately diverse with ToxoDB no. 2 (Type III) as the most frequent (15/20 = 75%) compared to other genotypes in Grenada.
- Subjects
GRENADA; TOXOPLASMA gondii; RESTRICTION fragment length polymorphisms; CHICKENS; POULTRY parasites
- Publication
Journal of Parasitology, 2017, Vol 103, Issue 1, p52
- ISSN
0022-3395
- Publication type
Article
- DOI
10.1645/15-945