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- Title
Cysteinyl Leukotrienes Induce Macrophage Inflammatory Protein-1 in Human Monocytes/Macrophages.
- Authors
Ichiyama, Takashi; Hasegawa, Masanari; Hashimoto, Kunio; Matsushige, Takeshi; Hirano, Reiji; Furukawa, Susumu
- Abstract
Background: Macrophage inflammatory protein-1α (MIP-1α) and MIP-1β are known for their chemotactic and proinflammatory effects on monocytes/macrophages which have a cysteinyl leukotriene 1 (CysLT1) receptor. Methods: We examined MIP-1α and MIP-1β production stimulated by CysLTs (LTC4, LTD4, and LTE4) in THP-1 cells, a human monocytic leukemia cell line, and peripheral blood mononuclear cells (PBMCs). Moreover, we examined the inhibitory effect of pranlukast, a CysLT1 receptor antagonist, and inhibitors of three major mitogen-activated protein kinases (MAPK) on the induction of MIP-1α and MIP-1β production by CysLTs. Results: ELISA demonstrated that CysLTs induced MIP-1α and MIP-1β production in THP-1 cells and PBMCs. PCR demonstrated that LTD4 increased MIP-1α and MIP-1β mRNA expressions in THP-1 cells. Pranlukast blocked MIP-1α and MIP-1β production promoted by LTD4 in THP-1 cells and PBMCs. Moreover, an inhibitor of extracellular signal-regulated kinase (ERK) attenuated the induction of MIP-1α and MIP-1β production by LTD4 in THP-1 cells whereas the inhibitors of c-Jun NH2-terminal kinase or p38 MAPK did not. Conclusion: CysLTs induce MIP-1α and MIP-1β production mediated by ERK via binding to the CysLT1 receptor in human monocytes/macrophages. Copyright © 2008 S. Karger AG, Basel
- Subjects
LEUKOTRIENES; ARACHIDONIC acid; EICOSANOIC acid derivatives; INFLAMMATORY mediators; CELL culture; ENZYME-linked immunosorbent assay
- Publication
International Archives of Allergy & Immunology, 2009, Vol 148, Issue 2, p147
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000155745