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- Title
In vitro reconstruction of tetronate RK-682 biosynthesis.
- Authors
Yuhui Sun; Hahn, Frank; Demydchuk, Yuliya; Chettle, James; Tosin, Manuela; Osada, Hiroyuki; Leadlay, Peter F.
- Abstract
The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein–bound substrates.
- Subjects
BIOSYNTHESIS; BIOCHEMISTRY; BIOCHEMICAL engineering; PHOSPHATASES; PROTEINS; GENE expression
- Publication
Nature Chemical Biology, 2010, Vol 6, Issue 2, p99
- ISSN
1552-4450
- Publication type
Article
- DOI
10.1038/nchembio.285