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- Title
Verification, Characterization and Tissue-specific Expression of UreG, a Urease Accessory Protein Gene, from the Amphioxus Branchiostoma belcheri.
- Authors
Ji-Yu XUE; Shi-Cui ZHANG; Nai-Guo LIU; Zhen-Hui LIU
- Abstract
UreG genes have been found in bacteria, fungi and plants but have not yet identified in animals, although a putative UreG-like gene has been documented in sea urchin. In the course of a large-scale sequencing of amphioxus gut cDNA library, we have identified a cDNA with high similarity to UreG genes. Both reverse transcription-polymerase chain reaction and nested polymerase chain reaction, as well as in situ hybridization histochemistry, verified that the cDNA represented an amphioxus UreG gene (AmphiUreG) rather than a microbial contaminant of the cDNA library. This is further supported by the presence of urease activity in amphioxus gut, gill and ovary. AmphiUreG encodes a deduced protein of 200 amino acid residues including a highly conserved P-loop, bearing approximately 46%-49%, 44%-48%, and 29%-37% similarity to fungal, plant and bacterial UreG proteins, respectively. It shows a tissue-specific expression pattern in amphioxus, and is especially abundant in the digestive system. This is the first UreG gene identified in animal species. Edited by Ming-Hua XU
- Subjects
UREASE; AMPHIOXUS; GENES; POLYMERASE chain reaction; SEA urchins; AMINO acids; PROTEINS
- Publication
Acta Biochimica et Biophysica Sinica, 2006, Vol 38, Issue 8, p549
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2006.00197.x