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- Title
Up-regulation ofNKX3.1Expression and Inhibition of LNCaP Cell Proliferation Induced by an Inhibitory Element Decoy.
- Authors
Jiang, An-Li; Hu, Xiao-Yan; Zhang, Peng-Ju; He, Mei-Lan; Kong, Feng; Liu, Zhi-Fang; Yuan, Hui-Qing; Zhang, Jian-Ye
- Abstract
NKX3.1is an androgen-regulated prostate-specific homeobox gene that is thought to play an important role in prostate development and cancerogenesis.NKX3.1acts as a tumor suppressor gene specifically in the prostate. Up-regulation ofNKX3.1gene offers a promising gene therapy for prostate cancer. The decoy strategy has been developed and is considered a useful tool for regulating gene expression and gene therapy. In our previous studies, we identified a 20 bp inhibitory element upstream of theNKX3.1promoter. In this study, we focused on using the 20 bp inhibitory element decoy to block negative regulation of theNKX3.1gene and to up-regulateNKX3.1expression using synthetic double-stranded oligodeoxynucleotides of the 20 bp inhibitory element. We found in an electrophoretic mobility shift assay experiment that the 20 bp inhibitory decoy presented competitive binding to a specific binding protein of the 20 bp inhibitory element in prostate cancer cell line LNCaP. In luciferase reporter gene assays, we found that the 20 bp inhibitory decoy could enhanceNKX3.1promoter activity, and RT-PCR and Western blot analysis revealed thatNKX3.1expression was up-regulated effectively by the transfection with the 20 bp inhibitory decoy. Furthermore, cell proliferation was inhibited by up-regulatedNKX3.1expression induced by the 20 bp inhibitory decoy.Edited byDing-Gan LIU
- Subjects
ANDROGENS; HOMEOBOX genes; PROSTATE; MORPHOGENESIS; CARCINOGENESIS; GENETIC regulation
- Publication
Acta Biochimica et Biophysica Sinica, 2005, Vol 37, Issue 5, p335
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2005.00047.x