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- Title
Utilization of Extracorporeal Membrane Oxygenation Alleviates Intestinal Ischemia-Reperfusion Injury in Prolonged Hemorrhagic Shock Animal Model.
- Authors
Zhao, Liang; Luo, Lin; Chen, Jinjin; Xiao, Juan; Jia, Weikun; Xiao, Yingbin
- Abstract
Intestinal ischemia-reperfusion injury is one of the main factors leading to multiple organ failure after resuscitation of prolonged hemorrhagic shock; however, the current conventional fluid resuscitation still cannot effectively reduce intestinal injury caused by prolonged hemorrhagic shock. To investigate the effect of ECMO resuscitation on alleviating intestinal ischemia-reperfusion injury in a prolonged hemorrhagic shock rabbit model. Thirty New Zealand white rabbits were randomly divided into three groups: control group, conventional fluid resuscitation group, and ECMO resuscitation group. The prolonged hemorrhagic shock model was established by keeping the arterial blood pressure from 31 to 40 mmHg for 3 h through the femoral artery bleeding, and performing the resuscitation for 2 h by conventional fluid resuscitation and ECMO resuscitation, respectively. Chiu's score of intestinal injury, serum lactate and TNF-α levels, intestinal mucosamyeloperoxidase (MPO) activity, intercellular adhesion molecule (ICAM-1), and Claudin-1expression were detected. The mean arterial blood pressure in Group 2 was significantly higher after resuscitation than in Group 1, but serum lactate and inflammatory cytokines TNF-α level were significantly lower. And Chiu's score of intestinal injury and myeloperoxidase (MPO) activity level and ICAM-1 expression were significantly lower in the ECMO resuscitation group, in which the Claudin-1 levels were significantly increased. ECMO resuscitation for the prolonged hemorrhagic shock improves tissue perfusion and reduces the systemic inflammation, and thus alleviates intestinal damage caused by prolonged hemorrhagic shock.
- Subjects
EXTRACORPOREAL membrane oxygenation; INTESTINAL ischemia; HEMORRHAGIC shock; MULTIPLE organ failure; RESUSCITATION; ANIMAL models in research
- Publication
Cell Biochemistry & Biophysics, 2014, Vol 70, Issue 3, p1733
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-014-0121-3