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- Title
Optimization of multi-enzyme cascade process for the biosynthesis of benzylamine.
- Authors
Jinli Wang; Runan Dong; Jingxin Yin; Jianhua Liang; Haijun Gao
- Abstract
Benzylamine is a valuable intermediate in the synthesis of organic compounds such as curing agents and antifungal drugs. To improve the efficiency of benzylamine biosynthesis, we identified the enzymes involved in the multi-enzyme cascade, regulated the expression strength by using RBS engineering in Escherichia coli, and established a regeneration-recycling system for alanine. This is a cosubstrate, coupled to cascade reactions, which resulted in E. coli RARE-TP and can synthesize benzylamine using phenylalanine as a precursor. By optimizing the supply of cosubstrates alanine and ammonia, the yield of benzylamine produced by whole-cell catalysis was increased by 1.5-fold and 2.7-fold, respectively, and the final concentration reached 6.21 mM. In conclusion, we achieved conversion from L -phenylalanine to benzylamine and increased the yield through enzyme screening, expression regulation, and whole-cell catalytic system optimization. This demonstrated a green and sustainable benzylamine synthesis method, which provides a reference and additional information for benzylamine biosynthesis research. The enzyme cascade in E. coli BL21 (DE3) RARE for the synthesis of benzylamine.
- Subjects
BENZYLAMINES; BENZYLAMINE; ORGANIC synthesis; ESCHERICHIA coli; BIOSYNTHESIS; ANTIFUNGAL agents
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 11, p1373
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbad111