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- Title
Cloning of a novel lectin from Artocarpus lingnanensis that induces apoptosis in human B-lymphoma cells.
- Authors
Luo, Yu; Liu, Xiaoqin; Lin, Faquan; Liao, Liejun; Deng, Yong; Zeng, Linjie; Zeng, Qiyan
- Abstract
We isolated a novel lectin (Artocarpus nitidus subsp. lingnanensislectin, ALL) fromArtocarpus nitidus subsp. lingnanensisand showed its mitogenic activities. In this study, we determined the amino acid sequence of ALL by cDNA sequencing.ALLcDNA (933 bp) contains a 657-bp open reading frame (ORF), which encodes a protein with 218 amino acids. ALL shares high sequence similarities with Jacalin and Morniga G and belongs to jacalin-related lectin family. We also examined the antitumor activity of ALL using Raji, a human B-lymphoma cell line. ALL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by N-acetyl-D-galactosamine (GalNAc). ALL inhibits Raji cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bcl-2 down-regulation, and caspase-3 activation. We further showed that the activation of p38 mitogen-activated protein kinase (MAPK) signaling pathways is required for the pro-apoptotic activity of ALL. ALL is a novel member of Jacalin-related lectins. ALL stimulates p38 MAPK signaling pathway and inhibits Raji cell proliferation through apoptosis.
- Subjects
CELL proliferation; REVERSE transcriptase polymerase chain reaction
- Publication
Bioscience, Biotechnology & Biochemistry, 2018, Vol 82, Issue 2, p258
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1415127