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- Title
Tenascin-C promotes migration of hepatic stellate cells and production of type I collagen.
- Authors
Ma, Jian-Cang; Su, Qing-Hua; Xu, Jin-Kai; Zhao, Jun; Huang, Xin; Shen, Ya-Wei; Zheng, Chen
- Abstract
Tenascin-C (TN-C) is an extracellular matrix glycoprotein markedly upregulated during liver fibrosis. The study is performed to explore the role of TN-C during the growth and activation of hepatic stellate cells (HSCs). We found that TN-C was accumulated accompanying with the HSC activation. Our data on cell migration assay revealed that the rTN-C treatment enhanced HSC migration in a dose- and time-dependent manner, but did not influence their proliferation. HSCs transfected with pTARGET-TN-C overexpression vector displayed increased the type I collagen (Col I) production. TN-C overexpression enhanced the process of HSC activation through TGF-β1 signaling. Moreover, the anti-α9β1 integrin antibody treatment blocked the TN-C-driven Col I increase in rat HSCs. Collectively, TN-C had a positive role in activation of HSCs mediated by TGF-β1 and α9β1 integrin, manifesting elevation of Col I production and promotion of cell migration. Our results provide a potential insight for the therapy of hepatic fibrosis. TN-C induced TGF-β1 and α9β1 expression to promote the cell migration and type I Collagen production in the activated HSCs.
- Subjects
TENASCIN; COLLAGEN; EXTRACELLULAR matrix
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 8, p1470
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1165600