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- Title
Subcellular mass determination by <sup>4</sup>He<sup>+</sup> energy-loss micro-spectrometry.
- Authors
Devès, Guillaume; Ortega, Richard
- Abstract
The scanning transmission ion microscope (STIM) has been used to determine the intracellular mass of human cultured cells. A 4He+ microbeam of 2.0 MeV energy was chosen to obtain enhanced ion-energy-loss sensitivity through the micron-thick freeze-dried cells. Local sample mass calculation, based on energy-loss conversion by use of appropriate matrix stopping powers, was performed by use of dedicated software. The method was validated with epoxy resin sections and polymer foil as analogues of biological samples in the range of (intra)cellular thickness, 150 to 3000 nm. STIM analysis resulted in less than 5% error in mass determination. 4He+ energy-loss micro-spectrometry was performed on freeze-dried human ovarian cancer cells, the mean areal mass obtained was 120 µg cm–2 (200 µg cm–2 in the nucleus and 250 µg cm–2 in nucleoli). This method is particularly useful for mass normalization of X-ray fluorescence yields resulting from particle-induced X-ray emission microanalysis (micro-PIXE). When performed successively these two ion-beam micro-analytical methods enable the mapping of true element concentrations within single cells.
- Publication
Analytical & Bioanalytical Chemistry, 2002, Vol 374, Issue 3, p390
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-002-1511-8