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- Title
Enzymatic and Chemoenzymatic Three-Step Cascades for the Synthesis of Stereochemically Complementary Trisubstituted Tetrahydroisoquinolines.
- Authors
Erdmann, Vanessa; Lichman, Benjamin R.; Zhao, Jianxiong; Simon, Robert C.; Kroutil, Wolfgang; Ward, John M.; Hailes, Helen C.; Rother, Dörte
- Abstract
Chemoenzymatic and enzymatic cascade reactions enable the synthesis of complex stereocomplementary 1,3,4-trisubstituted tetrahydroisoquinolines (THIQs) with three chiral centers in a step-efficient and selective manner without intermediate purification. The cascade employs inexpensive substrates (3-hydroxybenzaldehyde and pyruvate), and involves a carboligation step, a subsequent transamination, and finally a Pictet-Spengler reaction with a carbonyl cosubstrate. Appropriate selection of the carboligase and transaminase enzymes enabled the biocatalytic formation of (1 R,2 S)-metaraminol. Subsequent cyclization catalyzed either enzymatically by a norcoclaurine synthase or chemically by phosphate resulted in opposite stereoselectivities in the products at the C1 position, thus providing access to both orientations of the THIQ C1 substituent. This highlights the importance of selecting from both chemo- and biocatalysts for optimal results.
- Subjects
TETRAHYDROISOQUINOLINES; ENZYMATIC analysis; STEREOCHEMISTRY; SUBSTITUTION reactions; BIOCATALYSIS
- Publication
Angewandte Chemie, 2017, Vol 129, Issue 41, p12677
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201705855