We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Quantitative Measurement of Transmitters in Individual Vesicles in the Cytoplasm of Single Cells with Nanotip Electrodes.
- Authors
Li, Xianchan; Majdi, Soodabeh; Dunevall, Johan; Fathali, Hoda; Ewing, Andrew G.
- Abstract
The quantification of vesicular transmitter content is important for studying the mechanisms of neurotransmission and malfunction in disease, and yet it is incredibly difficult to measure the tiny amounts of neurotransmitters in the attoliter volume of a single vesicle, especially in the cell environment. We introduce a novel method, intracellular vesicle electrochemical cytometry. A nanotip conical carbon-fiber microelectrode was used to electrochemically measure the total content of electroactive neurotransmitters in individual nanoscale vesicles in single PC12 cells as these vesicles lysed on the electrode inside the living cell. The results demonstrate that only a fraction of the quantal neurotransmitter content is released during exocytosis. These data support the intriguing hypothesis that the vesicle does not open all the way during the normal exocytosis process, thus resulting in incomplete expulsion of the vesicular contents.
- Subjects
VESICLES (Cytology); CYTOPLASM; NEURAL transmission; CYTOMETRY; NEUROTRANSMITTERS; EXOCYTOSIS
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 41, p12146
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201504839