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- Title
Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks.
- Authors
Kim, Cheolhee; Lee, O-chul; Kim, Jae-Yeol; Sung, Wokyung; Lee, Nam Ki
- Abstract
Bending with high curvature is one of the major mechanical properties of double-stranded DNA (dsDNA) that is essential for its biological functions. The emergence of a kink arising from local melting in the middle of dsDNA has been suggested as a mechanism of releasing the energy cost of bending. Herein, we report that strong bending induces two types of short dsDNA deformations, induced by two types of local melting, namely, a kink in the middle and forks at the ends, which we demonstrate using D-shaped DNA nanostructures. The two types of deformed dsDNA structures dynamically interconvert on a millisecond timescale. The transition from a fork to a kink is dominated by entropic contribution (anti-Arrhenius behavior), while the transition from a kink to a fork is dominated by enthalpic contributions. The presence of mismatches in dsDNA accelerates kink formation, and the transition from a kink to a fork is removed when the mismatch size is three base pairs.
- Subjects
DNA derivatives; BENDING stresses; DNA structure; MECHANICAL behavior of materials; DEFORMATIONS (Mechanics)
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 31, p9071
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201502055