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- Title
Enhanced ajmalicine production in Catharanthus roseus cell cultures by combined elicitor treatment: from shake-flask to 20-l airlift bioreactor.
- Authors
Zhao, Jian; Zhu, Wei-Hua; Hu, Qiu
- Abstract
A two-stage process for enhanced ajmalicine production in elicited Catharanthus roseus cell cultures was developed in shake-flasks and a bioreactor. By using combined elicitor treatment of an Aspergillum niger mycelium and tetramethyl ammonium bromide, yields of ajmalicine were 48 mg l-1, 52 mg l-1 and 33 mg l-1, respectively in 500-ml flasks, 1000-ml flasks and a 20-l airlift bioreactor. The peroxidase and superoxide dismutase activities decreased in elicited cell cultures but catalase and lipoxygenase activities increased in these cultures. The combined elicitor treatment also caused a significant increase of malondialdehyde content in cell cultures.
- Subjects
CATHARANTHUS; APOCYNACEAE; CULTURES (Biology); CELL culture; MALONDIALDEHYDE; BIOREACTORS
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 6, p509
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005616920165