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- Title
L-精氨酸快速定量检测方法的研究.
- Authors
吕志飞; 王松江; 郭传庄; 隋松森; 李小双; 连战; 孙瑞成; 王建彬
- Abstract
The study was to optimize the optimal process parameters for determination of L-arginine in fermentation production process using an improved Sakuchi reagent method. Using L-arginine as test material, the effect of different detection wavelength, color development time, pH value and other conditions on determination of L-arginine content were investigated, and the reliability of detection method was explored. The results showed that the optimal detection conditions for the improved Sakuchi reagent method were 17.1 g/L sodium hydroxide solution in 7 mL, mixed chromogenic agent in 2 mL, centrifuged and appropriately diluted fermentation broth in 1 mL, water bath at 30 ℃ for 30 min before measuring OD525 nm value. The measured recovery rate of the sample was between 99.20% and 100.60%. The study indicates that the method has high sensitivity, good reproducibility, high stability, and can accurately and efficiently detect the content of L-arginine inproduction process of L-arginine fermentation.
- Publication
Feed Research, 2023, Vol 46, Issue 9, p130
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2023.09.026