We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
荆芥提取物抗UVA诱导HaCaT角质细胞的 光老化作用.
- Authors
黄立森; 陈芳芳; 庞海月; 吴黉坦; 王贵弘
- Abstract
The anti-photoaging efficacy of Schizonepeta tenuifolia extract (STE) was evaluated based on the photoaging model which was established by UVA irradiation upon HaCaT. The total phenolic content of STE was (111.09 ± 2.30) mg/g relative to gallic acid as determined by Folin-phenol Reagent. STE exerted significant antioxidant activity, indicative of the half scavenging concentration (SC50) of DPPH (1,1-diphenyl-2-picrylhydrazyl) of (0.18±0.04) mg/mL. The Trolox equivalent antioxidant activity of STE was (3.15 ± 0.06) mmol/g by ABTS (2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. In the enzyme linked immunosorbent assay (ELISA), STE could obviously reduce the production of intracellular reactive oxygen species (ROS) in HaCaT cells after UVA irradiation. STE could improve the cell survival rate after UVA irradiation as measured by Tetrazolium salt colorimetry (MTT). The results of Western blot showed that STE could effectively inhibit the expression of p-Histone H2AX-S139, p-atm, HSP70, Cleaved caspase-3, p-P38/MAPK and MMP-1 and increase the expression of Caspase-3 and Bcl-2 proteins compared with the vehicle-treated group. In conclusion, STE exerted obvious antiphotoaging activity by reducing the production of intracellular ROS, alleviating the ROS-induced intracellular DNA damage, antiapoptotic effect,and regulating the signaling pathways of heat shock proteins (HSP) and matrix metalloproteinase (MMP).
- Publication
Detergent & Cosmetics, 2021, Vol 44, Issue 10, p40
- ISSN
1006-7264
- Publication type
Article
- DOI
10.3969/j.issn.1006-7264.2021.10.007