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- Title
缺氧预处理对神经干细胞间隙连接蛋白的表达及半通道功能的影响.
- Authors
马伟康; 刘庆鹏; 刘 凯; 张 雷; 王立春; 韩 竹; 刘 亮; 李 琛; 孙崇毅
- Abstract
Determine the expression of gap junction protein and the changes of hemichannel function after hypoxia preconditioning of neural stem cells, which provided a theoretical basis for exploring the communication between neural stem cells after hypoxia preconditioning. Create a standardized preconditioning procedure and reproducibly expose the NSCs to hypoxia. Explore the best time for neural stem cells to be exposed to hypoxia without increasing necrosis. The expression of cx43, cx36, and cx47 in the neural stem cells of the control group and the hypoxia-preconditioned neurons were determined by immunohistochemical method at 1 h, 4 h, 8 h, and 12 h, respectively. The calcein dye efflux experiments was observed by laser confocal laser scanning microscopy to observe changes in hemichannell function. Compared with the control group (1.03+0.14%), the necrosis rate of neural stem cells in 8 hours (3.05+0.40%) and 12 hours (5.51+0.31%) exposed to 0.5% oxygen was significantly higher (P<0.05). Compared with the control group, the expression of cx43, Cx36 and Cx47 in neural stem cells increased significantly after hypoxic preconditioning for 4, 8 and 12 hours (P<0.05). Hypoxia pretreatment group had significantly enhanced hemichannel filtration function compared with the control group and the hemichannel blocker group. Hypoxic preconditioning can improve the expression of connexin and the half-channel filtration function of neural stem cells in a time-dependent manner. The optimal time for neural stem cells to be exposed to hypoxia without causing significant increase in necrosis is 4 hours.
- Subjects
NEURAL stem cells; GTPASE-activating protein; LASER microscopy; HYPOXEMIA; CONTROL groups
- Publication
Progress in Modern Biomedicine, 2020, Vol 20, Issue 8, p1420
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2020.08.005