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- Title
Decreased miR-218–5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer.
- Authors
Peng, Peng; Chen, Tao; Wang, Qing; Zhang, Yixi; Zheng, Fangfang; Huang, Shuai; Tang, Yubo; Yang, Chunxiao; Ding, Wenqing; Ren, Dong; Huang, Zongwen; Guo, Yuanqing
- Abstract
>bold<>italic<Background:>/italic<>/bold< miR-218–5p is an extensively studied microRNA (miRNA) in prostate cancer (PCa). However, the clinical significance and biological role of miR-218–5p in bone metastasis of PCa remain unclear. >bold<>italic<Materials and Methods:>/italic<>/bold< miR-218–5p expression was evaluated in 38 bone metastatic and 115 non-bone metastatic PCa tissues and serum samples. Clinical correlation of miR-218–5p expression with clinicopathological characteristics was analyzed. The biological roles of miR-218–5p in bone metastasis of PCa were investigated in vitro by invasion and migration assays. Bioinformatics analysis, real-time polymerase chain reaction, western blot, and luciferase reporter assay were applied to discern and examine the relationship between miR-218–5p and its potential targets. >bold<>italic<Results:>/italic<>/bold< miR-218–5p expression was reduced in bone metastatic PCa tissue and serum samples, which positively correlated with poor clinicopathological characteristics and bone metastasis-free survival in PCa patients. Upregulating miR-218–5p repressed PCa cell invasion and migration. Furthermore, miR-218–5p inhibited NF-κB signaling via simultaneously targeting TRAF1, TRAF2, and TRAF5, which suppressed the invasion and migration abilities of PCa cells. ROC curve analysis of miR-218–5p in the serum of PCa patients exhibited an area under the curve of 0.86 (95% confidence interval 0.80–0.92, p < 0.001). >bold<>italic<Conclusion:>/italic<>/bold< Our findings indicate that miR-218–5p might represent a novel serum biomarker for bone metastasis of PCa.
- Subjects
PROSTATE cancer; BONE metastasis; MICRORNA; SERUM; BLOOD serum analysis; BIOLOGICAL tags; POLYMERASE chain reaction
- Publication
Oncology Research & Treatment, 2019, Vol 42, Issue 4, p165
- ISSN
2296-5270
- Publication type
Article
- DOI
10.1159/000495473