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- Title
The Beneficial Effect of IL-12 and IL-18 Transduced Dendritic Cells Stimulated with Tumor Antigens on Generation of an Antitumor Response in a Mouse Colon Carcinoma Model.
- Authors
Mierzejewska, Jagoda; Węgierek-Ciura, Katarzyna; Rossowska, Joanna; Szczygieł, Agnieszka; Anger-Góra, Natalia; Szermer-Olearnik, Bożena; Geneja, Magdalena; Pajtasz-Piasecka, Elżbieta
- Abstract
The main purpose of our study was to determine the effect of dendritic cell (DC) transduction with lentiviral vectors carrying sequences of il18 and/or il12 genes on the level of antitumor activity in vitro and in vivo. We examined the ability of DCs to migrate to the tumor-draining lymph nodes and infiltrate tumor tissue and to activate the local and systemic antitumor response. On the 15th day, DCs genetically modified for production of IL-12 and/or IL-18 were administered peritumorally to C57BL/6 female mice with established MC38 tumors. Lymphoid organs and tumor tissue were collected from mice on the 3rd, 5th, and 7th days after a single administration of DCs for further analysis. Administration of DCs transduced for production of IL-12 alone and in combination with IL-18 increased the inflow and activity of CD4+ and CD8+ T lymphocytes in the tumor microenvironment and tumor-draining lymph nodes. We also found that even a single administration of such modified DCs could trigger a systemic antitumor response as well as inhibit tumor growth. Application of the developed DC-based vaccines may exert a favorable impact on stimulation of an antitumor immune response, especially if these DC vaccines are administered repeatedly.
- Subjects
TUMOR antigens; DENDRITIC cells; T cells; TUMOR growth; LYMPH nodes
- Publication
Journal of Immunology Research, 2022, p1
- ISSN
2314-8861
- Publication type
journal article
- DOI
10.1155/2022/7508928