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- Title
Effect of agitation and aeration on the production of nitrile hydratase by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor.
- Authors
Kamble, A. L.; Meena, V. S.; Banerjee, U. C.
- Abstract
Aims: To evaluate the effect of different physicochemical parameters such as agitation, aeration and pH on the growth and nitrile hydratase production by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. Methods and Results: Rhodococcus erythropolis MTCC 1526 was grown in 7-l reactor at different agitation, aeration and controlled pH. The optimum conditions for batch cultivation in the reactor were an agitation rate of 200 rev min−1, aeration 0·5 v/v/m at controlled pH 8. In this condition, the increase in nitrile hydratase activity was almost threefold compared to that in the shake flask. Conclusion: Agitation and aeration rate affected the dissolved-oxygen concentration in the reactor which in turn affected the growth and enzyme production. Significance and Impact of the Study: Cultivation of R. erythropolis MTCC 1526 in the reactor was found to have significant effect on the growth and nitrile hydratase production when compared to the shake flask.
- Subjects
MICROORGANISMS; MICROBIOLOGY; NOCARDIACEAE; ENZYMES; CATALYSTS
- Publication
Letters in Applied Microbiology, 2010, Vol 51, Issue 4, p413
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2010.02909.x