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- Title
The value of the local lymph node assay in quantitative structure-activity investigations.
- Authors
Basketier, D.A.; Rowerts, D.W.; Cronin, M.; Schols, E.W.
- Abstract
The development quantitative correlations between the physicochemical properties of compound and its ability to act as a skin sensitizer is complicated by the number of variables associated with the current sensitization test data, combined with the absence of a truly objective end point. Recently, however, a novel approach to the absence of skin sensitization potential, the local lymph node assay (LLNA), has been described, which determines the skin sensitization by measuring lymphocyte proliferation in lymph nodes draining the site of chemical exposure. The assay offers several advantages over traditional methods in the context of quantitative structure activity relationship studies. In the present work, a range of bromoalkanes has been employed which demonstrate the robustness and reproducibility of the LLNA. Sensitizing activity increased with chain length up to a maximum at C[sub15]/C[sub16] whereafter the response declined. The data were modelled against hydrophobicity, expressed as Clog P and (ClogP)[sup2] to fit the biphasic nature of the results. The results demonstrate the utility of LLNA data for interpretation in the context of quantitative structure-activity relationships, the limited number of variables, inter-test reproducibility) arid quantitative end point, lending themselves to mathematical interpretations
- Subjects
IMMUNOLOGY; QSAR models; LYMPH nodes; SKIN; ALLERGIES; BIOCHEMISTRY
- Publication
Contact Dermatitis (01051873), 1992, Vol 27, Issue 3, p137
- ISSN
0105-1873
- Publication type
Article
- DOI
10.1111/j.1600-0536.1992.tb05241.x