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- Title
In Vivo and Real-time Monitoring of Secondary Metabolites of Living Organisms by Mass Spectrometry.
- Authors
Bin Hu; Lei Wang; Wen-Cai Ye; Zhong-Ping Yao
- Abstract
Secondary metabolites are compounds that are important for the survival and propagation of animals and plants. Our current understanding on the roles and secretion mechanism of secondary metabolites is limited by the existing techniques that typically cannot provide transient and dynamic information about the metabolic processes. In this manuscript, by detecting venoms secreted by living scorpion and toad upon attack and variation of alkaloids in living Catharanthus roseus upon stimulation, which represent three different sampling methods for living organisms, we demonstrated that in vivo and real-time monitoring of secondary metabolites released from living animals and plants could be readily achieved by using field-induced direct ionization mass spectrometry.
- Subjects
CATHARANTHUS roseus; METABOLITES; METABOLOMICS; ORGANISMS; MASS spectrometry; CATHARANTHUS; BIOLOGICAL transport
- Publication
Scientific Reports, 2013, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep02104