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- Title
Nuclear Pore Complex Function in Saccharomyces cerevisiae Is Influenced by Glycosylation of the Transmembrane Nucleoporin Pom152p.
- Authors
Belanger, Kenneth D.; Gupta, Amitabha; MacDonald, Kristy M.; Ott, Christina M.; Hodge, Christine A.; Cole, Charles M.; Davis, Laura I.
- Abstract
The regulated transport of proteins across the nuclear envelope occurs through nuclear pore complexes (NPCs), which are composed of >30 different protein subunits termed nucleoporins. While some nucleoporins are glycosylated, little about the role of glycosylation in NPC activity is understood. We have identified loss-of-function alleles of ALG12, encoding a mannosyltransferase, as suppressors of a temperature-sensitive mutation in the gene encoding the FXFG-nucleoporin NUP1. We observe that nup1Δ cells import nucleophilic proteins more efficiently when ALG12 is absent, suggesting that glycosylation may influence nuclear transport. Conditional nup1 and nuf82 mutations are partially suppressed by the glycosylation inhibitor tunicamycin, while nic96 and nup116 alleles are hypersensitive to tunicamycin treatment, further implicating glycosylation in NPC function. Because Pom152p is a glycosylated, transmembrane nucleoporin, we examined genetic interactions between pom152 mutants and nup1Δ. A nup1 deletion is lethal in combination with pom152Δ, as well as with truncations of the N-terminal and transmembrane regions of Pom152p. However, truncations of the N-glycosylated, lumenal domain of Pom152p and pom152 mutants tacking N-linked glycosylation sites are viable in combination with nup1Δ, suppress nup1Δ temperature sensitivity, and partially suppress the nuclear protein import defects associated with the deletion of NUP1. These data provide compelling evidence for a role for glycosylation in influencing NPC function.
- Subjects
SACCHAROMYCES cerevisiae; GLYCOSYLATION; PROTEINS; GENETIC mutation; TEMPERATURE
- Publication
Genetics, 2005, Vol 171, Issue 3, p935
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.104.036319