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- Title
Metformin inhibits proliferation of human keratinocytes through a mechanism associated with activation of the MAPK signaling pathway.
- Authors
WEINING LI; WEIYUAN MA; HUA ZHONG; WENBIN LIU; QING SUN
- Abstract
In the present study, the effects of metformin on the proliferation of human immortalized keratinocytes (HaCaTs) and the underlying mechanisms were investigated. HaCaT cells in the logarithmic growth phase were treated with 50mMmetformin for 24, 48 and 72h. Cell morphology after 24h of treatment was observed under a microscope. Cell prolif¬eration was detected using a colorimetric cell proliferation and cytotoxicity assay kit. Western blot analyses were performed to detect the protein phosphorylation levels of adenosine monophosphate-activated protein kinase (AMPK) and extracellular signal-related kinase 1/2 (ERK1/2). Metformin treatment resulted in morphological changes of the HaCaT cells. The survival rates of HaCaT cells treated with metformin were 36.18, 12.70 and 10.12% at 24, 48 and 72h, respectively. As the treatment time extended, the survival rates of HaCaT cells decreased. Western blot analysis results showed that the mean level of phosphorylated (p)-AMPK in the HaCaT cells without metformin treatment was 2.856±0.323. However, the mean p-AMPK level following metformin treatment for 24h increased to 5.198±0.625, indicating a significant difference between these two groups (P<0.05). The mean absorbance ratio of p-ERK1/2 was 7.550±1.087 for the untreated cells, but the levels in cells following metformin treatment for 24 h increased to 10.430±1.217, indicating a significant difference between the two groups (P<0.05). In conclusion, metformin treatment upregulated the levels of p-AMPK and p-ERK1/2 in HaCaT cells, and significantly inhibited HaCaT cell prolifera¬tion in vitro by a mechanism associated with activation of the mitogen-activated protein kinase signaling pathway.
- Subjects
METFORMIN; KERATINOCYTES; PSORIASIS; ADENOSINE monophosphate; PROTEIN kinases; ADENOSINE kinase
- Publication
Experimental & Therapeutic Medicine, 2014, Vol 7, Issue 2, p389
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2013.1416