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- Title
Calcium-Activated Big-Conductance (BK) Potassium Channels Traffic through Nuclear Envelopes into Kinocilia in Ray Electrosensory Cells.
- Authors
Chen, Abby L.; Wu, Ting-Hsuan; Shi, Lingfang; Clusin, William T.; Kao, Peter N.
- Abstract
Electroreception through ampullae of Lorenzini in the little skate, Leucoraja erinacea, involves functional coupling between voltage-activated calcium channels (CaV1.3, cacna1d) and calcium-activated big-conductance potassium (BK) channels (BK, kcnma1). Whole-mount confocal microscopy was used to characterize the pleiotropic expression of BK and CaV1.3 in intact ampullae. BK and CaV1.3 are co-expressed in electrosensory cell plasma membranes, nuclear envelopes and kinocilia. Nuclear localization sequences (NLS) were predicted in BK and CaV1.3 by bioinformatic sequence analyses. The BK NLS is bipartite, occurs at an alternative splice site for the mammalian STREX exon and contains sequence targets for post-translational phosphorylation. Nuclear localization of skate BK channels was characterized in heterologously transfected HEK293 cells. Double-point mutations in the bipartite NLS (KR to AA or SVLS to AVLA) independently attenuated BK channel nuclear localization. These findings support the concept that BK partitioning between the electrosensory cell plasma membrane, nucleus and kinocilium may be regulated through a newly identified bipartite NLS.
- Subjects
CALCIUM-dependent potassium channels; NUCLEAR membranes; POTASSIUM channels; ALTERNATIVE RNA splicing; CALCIUM channels; PLASMA cells
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 17, p2125
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12172125