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- Title
Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field.
- Authors
Dey, Ranadhir; Alshaweesh, Jalal; Singh, Kamaleshwar P.; Lypaczewski, Patrick; Karmakar, Subir; Klenow, Laura; Paulini, Kayla; Kaviraj, Swarnendu; Kamhawi, Shaden; Valenzuela, Jesus G.; Singh, Sanjay; Hamano, Shinjiro; Satoskar, Abhay R.; Gannavaram, Sreenivas; Nakhasi, Hira L.; Matlashewski, Greg
- Abstract
The leishmanin skin test was used for almost a century to detect exposure and immunity to Leishmania, the causative agent of leishmaniasis, a major neglected tropical disease. Due to a lack of antigen used for the intradermal injection, the leishmanin skin test is no longer available. As leishmaniasis control programs are advancing and new vaccines are entering clinical trials, it is essential to re-introduce the leishmanin skin test. Here we establish a Leishmania donovani strain and describe the production, under Good Laboratory Practice conditions, of leishmanin soluble antigen used to induce the leishmanin skin test in animal models of infection and vaccination. Using a mouse model of cutaneous leishmaniasis and a hamster model of visceral leishmaniasis, soluble antigen induces a leishmanin skin test response following infection and vaccination with live attenuated Leishmania major (LmCen-/-). Both the CD4+ and CD8+ T-cells are necessary for the leishmanin skin test response. This study demonstrates the feasibility of large-scale production of leishmanin antigen addressing a major bottleneck for performing the leishmanin skin test in future surveillance and vaccine clinical trials. As leishmaniasis control programs and new vaccines are advancing, it is necessary to re-introduce the leishmanin skin test to monitor transmission and immunity. This study describes the generation and validation of a new leishmanin skin test antigen for future re-introduction into endemic countries.
- Subjects
LEISHMANIA donovani; ANTIGEN analysis; SKIN tests; LEISHMANIA; VACCINE trials; VISCERAL leishmaniasis; CUTANEOUS leishmaniasis
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-42732-2