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- Title
Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density.
- Authors
Prin, L.; Charon, J.; Capron, M.; Gosset, Ph.; Taelman, H.; Tonnel, A. B.; Capron, A.
- Abstract
Recent work has suggested that there may be heterogeneity amongst human eosinophils. In order to study this further, blood and tissue eosinophils were separated by density gradient centrifugation in discontinuous gradients of metrizamide. Blood eosinophils were obtained from 16 patients with blood eosinophil counts above 1 × 109/1. Resident eosinophils were isolated from alveolar fluid in three of these patients were of light buoyant density (hypodense) and a high percentage of these showed morphological evidence of hypogranulation. Hypodense eosinophils had a reduced capacity to reduce nitroblue tetrazolium or develop chemiluminescence after stimulation with phorbol myristate acetate. It was concluded that there is structural and metabolic heterogeneity amongst blood and tissue eosinophils in patients with a variety of eosinophilic disorders.
- Subjects
EOSINOPHILS; METRIZAMIDE; TETRAZOLIUM; CHEMILUMINESCENCE; PATIENTS; GRANULOCYTES
- Publication
Clinical & Experimental Immunology, 1984, Vol 57, Issue 3, p735
- ISSN
0009-9104
- Publication type
Article