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- Title
Biofilm formation by Bacillus subtilis requires an endoribonuclease-containing multisubunit complex that controls mRNA levels for the matrix gene repressor SinR.
- Authors
DeLoughery, Aaron; Dengler, Vanina; Chai, Yunrong; Losick, Richard
- Abstract
Biofilm formation by B acillus subtilis is largely governed by a circuit in which the response regulator Spo0A turns on the gene for the anti-repressor SinI. SinI, in turn, binds to and inactivates SinR, a dedicated repressor of genes for matrix production. Mutants of the genes ylb F, ymc A and yaa T are blocked in biofilm formation, but the mechanism by which they act has been mysterious. A recent report attributed their role in biofilm formation to stimulating Spo0A activity. However, we detect no measurable effect on the transcription of sin I. Instead, we find that the block in biofilm formation is caused by an increase in the levels of SinR and of its m RNA. Evidence is presented that YlbF, YmcA and YaaT interact with, and control the activity of, RNase Y, which is known to destabilize sin R m RNA. We also show that the processing of another target of RNase Y, cgg R- gap A m RNA, similarly depends on YlbF and YmcA. Our work suggests that sin R m RNA stability is an additional posttranscriptional control mechanism governing the switch to multicellularity and raises the possibility that YlbF, YmcA and YaaT broadly regulate m RNA stability as part of an RNase Y-containing, multi-subunit complex.
- Subjects
BACILLUS subtilis; BIOFILMS; ENDORIBONUCLEASES; MESSENGER RNA; GENETIC repressors; RNA interference
- Publication
Molecular Microbiology, 2016, Vol 99, Issue 2, p425
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.13240