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- Title
Local and Systemic Induction of β-1,3-Glucanase and Chitinase in Coffee Leaves Protected Against <em>Hemileia vastatrix</em> by <em>Bacillus thuringiensis</em>.
- Authors
Guzzo, S. D.; Martins, E. M. F.
- Abstract
β-l,3-glucanase and chitinase activities were induced locally and systemically 4-25 and 11-25 days, respectively, after spraying the surface of the third pair of coffee leaves from the apex of 8-month-old plants with a 50 mg/ml aqueous suspension of Bacillus thuringiensis in a commercial formulation (Thuricide HP-Sandoz). The treatment also induced local and systemic resistance against Hemileia vastatrix after the application of the inducer. Within 14-18 days of application of the Thuricide inducer, the β-l ,3-glucanase activity in the locally- and systemically-protected unchallenged leaves reached maximum levels of 226% and 279% higher levels respectively, than in control plants. The chitinase activity reached maximum levels of 224% and 181% respectively, within 18-21 days after treatment with the inducer. Two β-l,3-glucanase bands were detected by native PAGE electrophoresis in extracts from locally-and systemically- protected unchallenged coffee leaves.
- Subjects
COFFEE; CHITINASE; GLYCOSIDASES; HEMILEIA vastatrix; BACILLUS thuringiensis; BACILLUS (Bacteria)
- Publication
Journal of Phytopathology, 1996, Vol 144, Issue 9/10, p449
- ISSN
0931-1785
- Publication type
Article
- DOI
10.1111/j.1439-0434.1996.tb00322.x