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- Title
A High-Performance Liquid Chromatography with Photodiode Array Detection Method for Simultaneous Determination of Three Compounds Isolated from Wikstroemia ganpi : Assessment of the Effects on Cytochrome P450-Mediated Metabolism In Vitro and In Vivo.
- Authors
Keem, Min-Ji; Seo, Seong-Wook; Kim, Taeyoung; Jo, Beom-Geun; Kim, Su-Nam; Yoon, In-Soo; Yang, Min Hye
- Abstract
In natural products, the content and quality of the marker components differ depending on the part, production area, collection period, and extraction method; therefore, a standardized analysis method is required to obtain consistent results. This study developed a simultaneous analysis method for three marker components (7-methoxylutolin-5-O-glucoseide, pilloin 5-O-β-d-glucopyranoside, rutarensin) isolated and purified from Wikstroemia ganpi (W. ganpi). Simultaneous analysis was performed using high-performance liquid chromatography with photodiode array detection (HPLC-PDA) method that was validated according to the International Council for Harmonisation (ICH) guidelines. The developed analytical method exhibited linearity (r2 > 0.999), detection limits (0.72–3.34 μg/mL), and quantification limits (2.19–10.22 μg/mL). The relative standard deviation (RSD) value of intra- and inter-day precisions was less than 1.68%, and analyte recoveries (93.42–117.55%; RSD < 1.86%) were validated according to the analytical procedures, and all parameters were within the allowable range. Quantitative analysis of the three marker components from W. ganpi MeOH extract (WGM) showed 7-methoxylutolin-5-O-glucoseide with the highest content (51.81 mg/g). The inhibitory effects of WGM on cytochrome P450 (CYP) substrate drugs were further investigated. The in vitro study revealed that WGM inhibited the CYP3A-mediated metabolism of buspirone and that 7-methoxylutolin-5-O-glucoseide and pilloin 5-O-β-d-glucopyranoside inhibited the metabolism of buspirone with IC50 values of 2.73 and 18.7 μM, respectively. However, a single oral dose of WGM did not have significant effects on the pharmacokinetics of buspirone in rats, suggesting that WGM cannot function as an inhibitor of CYP3A-mediated metabolism in vivo.
- Subjects
TISSUE arrays; IN vitro studies; MEDICINAL plants; HIGH performance liquid chromatography; CYTOCHROME P-450; IN vivo studies; METHANOL; ORAL drug administration; ANIMAL experimentation; METABOLISM; QUANTITATIVE research; RATS; RESEARCH funding; PLANT extracts; CHEMICAL inhibitors
- Publication
Nutrients, 2023, Vol 15, Issue 18, p4061
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15184061