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- Title
Inulin Prebiotic Protects against Lethal Pseudomonas aeruginosa Acute Infection via γδ T Cell Activation.
- Authors
Boucher, Emilie; Plazy, Caroline; Le Gouellec, Audrey; Toussaint, Bertrand; Hannani, Dalil
- Abstract
Pseudomonas aeruginosa (P. aeruginosa) causes harmful lung infections, especially in immunocompromised patients. The immune system and Interleukin (IL)-17-producing γδ T cells (γδ T) are critical in controlling these infections in mice. The gut microbiota modulates host immunity in both cancer and infection contexts. Nutritional intervention is a powerful means of modulating both microbiota composition and functions, and subsequently the host's immune status. We have recently shown that inulin prebiotic supplementation triggers systemic γδ T activation in a cancer context. We hypothesized that prophylactic supplementation with inulin might protect mice from lethal P. aeruginosa acute lung infection in a γδ T-dependent manner. C57Bl/6 mice were supplemented with inulin for 15 days before the lethal P. aeruginosa lung infection, administered intranasally. We demonstrate that prophylactic inulin supplementation triggers a higher proportion of γδ T in the blood, accompanied by a higher infiltration of IL-17-producing γδ T within the lungs, and protects 33% of infected mice from death. This observation relies on γδ T, as in vivo γδ TcR blocking using a monoclonal antibody completely abrogates inulin-mediated protection. Overall, our data indicate that inulin supplementation triggers systemic γδ T activation, and could help resolve lung P. aeruginosa infections. Moreover, our data suggest that nutritional intervention might be a powerful way to prevent/reduce infection-related mortality, by reinforcing the microbiota-dependent immune system.
- Subjects
BIOLOGICAL models; LUNG injuries; PREBIOTICS; IN vivo studies; ANIMAL experimentation; MONOCLONAL antibodies; PSEUDOMONAS diseases; HUMAN microbiota; INTRANASAL administration; T cells; DEATH; INULIN; ACUTE diseases; MICE
- Publication
Nutrients, 2023, Vol 15, Issue 13, p3037
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15133037