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- Title
EVALUATION OF GENETIC DIVERSITY IN OPEN POLLINATED GUAVA BY iPBS PRIMERS.
- Authors
Mehmood, Asim; Jaskani, Muhammad Jafar; Ahmad, Saeed; Ahmad, Rashid
- Abstract
DNA markers are important tools for assessing genetic diversity and relationships among species, cultivars and breeding materials. Many horticultural species are lacking genomic information. DNA markers that do not require prior knowledge of DNA sequences are therefore appealing for horticultural research. A retrotransposon-based DNA marker system, iPBS (inter primer binding sites) developed from conserved primer binding sites within retrotransposons, was used to study the genetic variation and relationships in ornamental guava. PCR from 6 iPBS primers (dominant markers) produced a total of 113 bands (52.38-100% polymorphic) ranging from 150 bp to 3000 bp, and the mean PIC value for each primer ranging from 0.1245 to 0.3698. Molecular information generated from both iPBS was separately scored in a matrix for phylogenetic dendrogram construction. The phylogenetic dendrogram based on iPBS markers reflected morphologic classifications of the accessions that were studied. The iPBS PCR-based genome fingerprinting technology in this study is low-cost and provides another effective alternative in differentiation of accessions in guava (Psidium guajava Linn.) and related species or genera.
- Subjects
PLANT species diversity; GUAVA; DNA primers; GENETIC markers in plants; HORTICULTURAL crops; DNA fingerprinting of plants
- Publication
Pakistan Journal of Agricultural Sciences, 2013, Vol 50, Issue 4, p591
- ISSN
0552-9034
- Publication type
Article