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- Title
Overexpressing the glutaredoxin (CaGrx) gene enhances the antioxidant defences and improves drought tolerance in chickpea (Cicer arietinum L.).
- Authors
Kumar, Varun; Kumar, Anil; Meenakshi; Gupta, Uma; Narayan, Shiv; Shirke, Pramod Arvind; Sanyal, Indraneel
- Abstract
Rising global temperatures are expected to worsen the impact of drought, which directly impacts the worldwide production of chickpea. Glutaredoxins (Grxs) are ubiquitous, heat-stable, cysteine-rich proteins and glutathione-dependent thiol-disulfide oxidoreductases. The overexpression of the CC-type Grxs gene in plants improves drought and salinity stress tolerance. The present study reports the potential role of chickpea glutaredoxin (CaGrx) gene in response to drought stress. The CaGrx gene from chickpea was overexpressed in Kabuli-type chickpea by the Agrobacterium-mediated gene transformation method. Integration of the CaGrx gene in the Kabuli chickpea genome was confirmed by PCR using gene-specific primers. The qRT-PCR showed an 11- to 14-fold change in the expression of the CaGrx transcript in the T1 generation of transgenic chickpea. The T1 generation of the transgenic chickpea exhibit improved tolerance to drought stress due to the enhanced activities of different antioxidant enzymes such as glutaredoxin (GRX), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT), and reduced levels of stress markers H2O2 and thiobarbituric acid reactive substances (TBARS). Also, the CaGrx overexpression lines of T1 generation showed improved physiological performance, including net photosynthesis (PN), transpiration (E), water use efficiency (WUE), stomatal conductance (gs), PSII (Fv/Fm), and non-photochemical quenching (NPQ) during drought, which help to maintain the photosynthetic apparatus and protect the plants from oxidative damage. Overall, the study showed that the overexpression of CaGrx improves the ability of chickpea to withstand drought, and the CaGrx gene could be used to develop drought-tolerant crop plants. Key message: Overexpression of the chickpea glutaredoxin (CaGrx) gene in chickpea enhances drought tolerance by enhancing antioxidant enzymes defense, reducing oxidative damage, and boosting plant growth and productivity under drought stress conditions.
- Subjects
CHICKPEA; DROUGHT tolerance; GLUTAREDOXIN; WATER efficiency; PLANT genes; GLUTATHIONE reductase
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 156, Issue 2, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-023-02651-y