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- Title
Isolation and characterization of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul ( Phyllastrephus flavostriatus) and cross-species amplification in four closely related taxa.
- Authors
LOKUGALAPPATTI, L. G. SAMPATH; FELDHEIM, KEVIN A.; SELLAS, ANNA B.; BOWIE, RAURI C. K.
- Abstract
We describe the isolation of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul using an enrichment protocol. All loci were highly variable with the number of alleles ranging from 8 to 13, and observed heterozygosity ranging from 0.652 to 0.870. All loci were in Hardy–Weinberg equilibrium; however, loci Pfl12 and Pfl54 showed significant linkage disequilibrium. All 10 loci successfully amplified and were polymorphic in at least one of four related Phyllastrephus species. These loci should prove to be widely applicable to studies of phylogeography, hybridization and paternity in African greenbuls.
- Subjects
PHYLLASTREPHUS flavostriatus; GREENBULS; MICROSATELLITE repeats; HARDY-Weinberg formula; GENETIC polymorphisms; ANIMAL species; SPECIES hybridization
- Publication
Molecular Ecology Resources, 2008, Vol 8, Issue 3, p622
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1471-8286.2007.02023.x